Composite

Part:BBa_K2443000:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-04)


AlaRS optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 720
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2524
    Illegal BsaI site found at 2701
    Illegal BsaI.rc site found at 1073
    Illegal SapI site found at 1808


Design Notes

Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli



Source

see basic parts

References