Composite
Part:BBa_K2443000:Design
Designed by: Lethbridge iGEM 2017 Group: iGEM17_Lethbridge (2017-10-04)
AlaRS optimized for expression in E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 513
Illegal BglII site found at 720
Illegal BamHI site found at 686 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1124
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2524
Illegal BsaI site found at 2701
Illegal BsaI.rc site found at 1073
Illegal SapI site found at 1808
Design Notes
Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli
Source
see basic parts